Improving Product Added-value from Shrimp (Litopenaeus vannamei) Waste by Using Enzymatic Hydrolysis and Response Surface Methodology
2021
Nguyen, Nguyen Van | Hai, Pham Duy | My My, Vo Thi | Men, Dinh Thi | Trung, Le Duc | Bavor, H John
Improving product added-value from shrimp wastes and reducing environmental pollution are important to the shrimp aquaculture industry. Response surface methodology was employed to optimize the ratio of enzyme to the substrate (ES), temperature, time, pH to maximize hydrolysis and soluble protein (SP) content from shrimp liquid waste hydrolysate. Optimal hydrolysis conditions to obtain maximum DH (27.90%) and SP (59.76 mg/g) were ES (0.21%), temperature (58.87), time (67.76 min), and pH (7.87). Characteristic analysis of the product indicated that the hydrolysate had high chemical scores of essential amino acids for humans (0.98), tilapia (1.16), and rainbow trout (1.52), flavor amino acid-rich protein (51.96 g/100 g of protein), and total carotenoids (330.5 μg/g). Previous studies have not considered value and environmental benefits achieved through utilization of shrimp liquid waste. The results indicate that shrimp liquid waste hydrolysate can be used as a valuable feed supplement and feed attractant for aquaculture.
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