In vitro growth and shoot multiplication of Wrightia tomentosa Roem et Schult in a controlled carbon dioxide environment
2003
Vyas, S. | Purohit, S.D.
In vitro growth and multiplication of shoots of a woody tree species Wrightia tomentosa in a controlled carbon dioxide environment was studied. The cultures were grown on BA supplemented MS medium with or without 3% sucrose. A range of CO2 concentrations (0.0, 0.6, 10.0 and 40.0 g m-3) was controlled in small chambers by using solutions of NaHCO3, Na2CO3, KHCO3 and K2CO3. To obtain a CO2-free environment, a saturated solution of KOH was kept in the chambers. It was concluded that the growing shoot cultures required either sucrose in the medium as a carbon source or an ambient CO2 environment. Complete absence of a carbon source caused severe browning of the shoots and death within 30 days. The cultures grew better with 10.0 g m-3 carbon dioxide in the environment than with 3.0% sucrose in the medium. With both CO2 and sucrose being available, the best response was obtained at 0.6 g m-3 CO2 in the chamber. At this concentration the rate of shoot multiplication was nearly double the standard rate obtained when exposed to the natural CO2 level and sucrose-supplemented medium. Total fresh and dry weight, leaf number and area per cluster also showed the best response under this condition.
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