Rapid alpha-amylase assays for assessment of pre-harvest sprouting damage in wheat
1989
Henry, R.J.
Rapid specific alpha-amylase assays were developed for use in the assessment of pre-harvest sprouting damage in wheat. Two different commercially available alpha-amylase substrates, an insoluble cross-linked dye-labelled starch and a soluble blocked p-nitrophenyl maltoheptaoside, were investigated. Extraction of alpha-amylase from ground wheat was shown to be very rapid. Assays were simplified and speeded up by including the substrates in the extraction medium. Action on the substrates in the extraction medium for 2 min was used to estimate alpha-amylase activity. Results for the rapid methods correlated well with those obtained in assays with separate extraction and assay steps and the Falling Number test. The assay with the insoluble substrate could be conducted at higher temperatures and was thus more sensitive. These assay procedures have potential for application in assessing sprouting damage for segregation of damaged wheat in commercial production.
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