Genetic and physical fine mapping of Scmv2, a potyvirus resistance gene in maize
2010
Ingvardsen, Christina Roenn | Xing, Yongzhong | Frei, Ursula Karoline | Lübberstedt, Thomas
Sugarcane mosaic virus (SCMV) is an important virus pathogen both in European and Chinese maize production, causing serious losses in grain and forage yield in susceptible cultivars. Two major resistance loci confer resistance to SCMV, one located on chromosome 3 (Scmv2) and one on chromosome 6 (Scmv1). We developed a large isogenic mapping population segregating in the Scmv2, but not the Scmv1 region, to minimize genetic variation potentially affecting expression of SCMV resistance. We fine mapped Scmv2 to a region of 0.28 cM, covering a physical distance of 1.3426 Mb, and developed six new polymorphic SSR markers based on publicly available BAC sequences within this region. At present, we still have three recombinants left between Scmv2 and the nearest polymorphic marker on either side of the Scmv2 locus. The region showed synteny to a 1.6 Mb long sequence on chromosome 12 in rice. Analysis of the public B73 BAC library as well as the syntenic rice region did not reveal any similarity to known resistance genes. However, four new candidate genes with a possible involvement in movement of virus were detected.
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