Structural and expression analysis of prolamin genes in Oryza sativa L
2012
Yoon, Ung-Han | Lee, Jeonghwa | Hahn, Jang-Ho | Kim, Yeon-Ki | Lee, Gang-Seob | Ji, Hyeon-So | Kim, Chang-Kug | Mun, Jeong-Hwan | Kim, Young Mi | Kim, Tae-Ho
Rice is a staple crop with a small genome of 389Â Mb. Rice grain is a source of carbohydrates and proteins and has a relatively low protein content compared to other legume seeds. Glutelin and prolamin are the major storage proteins in rice. Prolamins are characterized by high glutamine and proline content and are generally soluble only in strong alcohol solutions. In this study, we obtained a total of 51,383 expressed sequence tags (ESTs) from Ilpumbyeo (Oryza sativa L.), of which 33,201 and 18,182 clones were obtained from immature and germinating seeds, respectively. From the EST clones, 15,148 unigenes were identified, and 2,590 genes were expressed in both immature and germinating seeds. Gene expression profiling of rice prolamins indicated that prolamin gene expression increased 5Â days after heading and reached maximal expression after 30Â days, suggesting a high demand for prolamins during seed development and germination. Phylogenetic analysis grouped 33 prolamin genes based on the abundance of sulfur-containing amino acids methionine and cysteine according to the deduced amino acid sequences. Our results enhance the understanding of the regulation of seed maturation and germination, which can result in improved agricultural traits for the seed industry.
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