High production of xanthan gum by a glycerol-tolerant strain Xanthomonas campestris WXLB-006
2017
Wang, Zichao | Wu, Jianrong | Gao, Min-Jie | Zhu, Li | Zhan, Xiao-Bei
The superior properties of xanthan gum make it an industrial aginomoto used in many industries, especially in oil recovery. In the present work, xanthan production from glycerol by a mutant strain Xanthomonas campestris WXLB-006 reached as high as 17.8 g/L in flask culture. With the adoption of pH control, varied aeration and agitation, and varied glycerol feeding strategy, xanthan production reached 33.9 g/L in a 7-L fermenter and fermentation time decreased to 60 hr. Instead of difficultly and costly purifying glycerol, this research provides a very good case for glycerol utilization. At the same time, this is the first report on a high glycerol-tolerant strain for microbial polysaccharide production and 33.9 g/L is the highest production of xanthan gum produced from glycerol so far.
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