A potential role of green engineered TiO2 nanocatalyst towards enhanced photocatalytic and biomedical applications

This study demonstrates a simple protocol for phytofabrication of titanium dioxide nanoparticles (TiO₂NPs) wrapped with bioactive molecules from Ludwigia octovalvis leaf extract and their characterization by UV-visible absorption spectroscopy, Fourier transform spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), X-ray photoelectron spectrum (XPS), and diffuse reflectance spectrum (DRS). The bandgap energy of pure green engineered TiO₂ nanoparticles was determined by DRS analysis. The XPS analysis confirmed the purity of the TiO₂ nanoparticles. Results show that the synthesized TiO₂NPs were spherical in shape with the size ranged from 36 to 81 nm. The green engineered titanium oxide nanocatalyst exhibited enhanced rate of photocatalytic degradation of important textile toxic dyes namely crystal violet (93.1%), followed by methylene blue (90.6%), methyl orange (76.7%), and alizarin red (72.4%) after 6-h exposure under sunlight irradiation. Besides, this study determines the antimicrobial efficiency of TiO₂NPs (25 μl and 50 μl), leaf extract (25 μl), and antibiotic (25 μl) against clinically isolated human pathogenic bacterial strains namely Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus epidermidis, and Escherichia coli. Results show that maximum antibacterial activity with nanotitania treatment noticed was 21.6 and 18.3-mm inhibition in case of S. epidermis and P. aeruginosa, respectively. Enhanced rate of antibiofilm activity towards S. aureus and K. pneumoniae was also observed with TiO₂NPs exposure. The biomolecule loaded TiO₂NPs exhibited the fastest bacterial deactivation dynamics towards gram-negative bacteria (E. coli), with a complete bacterial inactivation within 105-min exposure. Interestingly, anticancer activity result indicates that percentage of human cervical carcinoma cell (HeLa) viability was negatively correlated with TiO₂NPs doses used. The AO/EtBr fluorescent staining result exhibited the occurrence of more apoptosis (dead cells) of HeLa cells due to the exposure of TiO₂NPs. Altogether, the present study clearly showed that biomolecules wrapped nanotitania could be used as effective and promising compound for enhanced photocatalytic and biomedical applications in the future.