Cellulose Synthase-Like CslF Genes Mediate the Synthesis of Cell Wall (1,3;1,4)-{szligbeta}-D-Glucans
Burton, Rachel A. | Wilson, Sarah M. | Hrmova, Maria | Harvey, Andrew J. | Shirley, Neil J. | Medhurst, Anne | Stone, Bruce A. | Newbigin, Edward J. | Bacic, A (Antony) | Fincher, Geoffrey B.
A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-{szligbeta}-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-{szligbeta}-D-glucan content in barley grain to a cluster of cellulose synthase-like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-{szligbeta}-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-{szligbeta}-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-{szligbeta}-D-glucan biosynthesis.
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