Isolation, identification and characterisation of a soil bacterium producing an enzyme with L-phenylalanine oxidase activity
1994
Brearley, G.M. | Price, C.P. | Atkinson, T. | Hammond, P.M.
A gram-positive, mesophilic bacterium which assimilated L-phenylalanine but which failed to utilise L-tyrosine was isolated from soil. The isolate, identified as a strain of Bacillus carotarum, converted L-phenylalanine to phenylpyruvate with the initial step catalysed by an inducible. intracellular enzyme which possessed L-phenylalanine oxidase activity. Phenylalanine oxidase has not been previously reported in Gram-positive bacteria, although there are a few examples of non-specific L-amino acid oxidases with activity towards L-phenylalanine. The isolate grew abundantly on complex media but failed to synthesize significant amounts of the enzyme in the absence of L-phenylalanine. The highest enzyme levels were achieved in a chemically defined minimal salts medium containing the amino acid at 10 g/l as the primary carbon and energy source.
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