Quantification of Vitamin A in Palm Oil Using a Fast and Simple Portable Device: Method Validation and Comparison to High-Performance Liquid Chromatography
Rohner, Fabian | Frey, Simone K. | Mothes, R. (Ralf) | Hurtienne, Andrea | Hartong, Simone | Bosso, Patrice Emery | Mai, Bui | Schweigert, Florian J. | Northrop-Clewes, Christine
Vitamin A deficiency continues to be a global public health problem. Fortification of oil with vitamin A is considered a cost-effective, feasible strategy to prevent this problem but quality control poses a challenge to program implementation. To overcome this, we have validated a newly developed device that quantitatively measures the content of retinyl palmitate in refined palm oil, is simple to use, and yields immediate results.Linearity of analysis ranged from 2.5 - 30 mg retinol equivalents (RE)/ kg of palm oil, with 2.5 mg RE/kg being the determination limit; inter- and intra-assay precision ranged from 1.4 - 7.1 %. Comparison with a high-performance liquid chromatography method showed high agreement between the methods (R<sup>2</sup> = 0.92; Limits of Agreement: -1.24 mg to 2.53 mg RE/kg), and further comparisons illustrate that the new device is useful in low-resource settings. This device offers a field- and user-friendly solution to quantifying the vitamin A content in refined palm oil.
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