Development of a Highly Sensitive Colorimetric Method for Detecting 17β-Estradiol Based on Combination of Gold Nanoparticles and Shortening DNA Aptamers

Pu, Hongbin; Huang, Zhibin; Sun, Da-Wen; Xie, Xiaohui; Zhou, Weibiao

Development of a Highly Sensitive Colorimetric Method for Detecting 17β-Estradiol Based on Combination of Gold Nanoparticles and Shortening DNA Aptamers

2019

Pu, Hongbin | Huang, Zhibin | Sun, Da-Wen | Xie, Xiaohui | Zhou, Weibiao

Pollution of environmental endocrine disruptors has caused increasing concern globally. In the current study, a simple colorimetric method with high sensitivity and good selectivity for 17β-estradiol (E2) detection was developed, which employed gold nanoparticles (AuNPs) as colorimetric probe for specific recognition of shortening DNA aptamer. Visible color change from bright red to violet was observed for aggregation of AuNPs without the protection of DNA aptamer. After optimization, the method exhibited great performance for E2 detection with good linearity between E2 concentrations from 0.2 to 5 nM and the absorbance ratio at 620 and 523 nm, with the limit of detection of 0.1 nM. The method was also successfully applied to E2 determination in different spiked water samples including fishpond water, lake water, and tap water, in which good recoveries from 93.1 to 108.9% and acceptable relative standard deviations from 3.4 to 8.9% were obtained. This technique showed great potential for on-site fast determination of E2 in environmental water samples.

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