The fatty acid composition and Na+-K+ ATPase activity of kidney microsomes from mice consuming diets of varying docosahexaenoic acid and linoleic acid ratios
Male mice (CD-1 type) were fed for 14 days diets containing 4 grams of ethyl esters of linoleic acid (LA) per 100 g of diet supplemented with 0, 0.4, 0.8 or 4 g of ethyl ester of docosahexaenoic acid (DHA). The total fat content of the diets was adjusted to 10% by weight by adding a 1:1 mixture of tristearin and triolein. Kidney microsomes from these mice were prepared and analysed for fatty acid content and Na+-K+ATPase activity. Feeding the lowest amount of DHA, representing a 1:10 dietary DHA/LA ratio, resulted in an increase in DHA content from 6.5 to 17 mole % of phospholipids from microsomes compared to diets without DHA. Higher dietary DHA levels did not result in further increases in tissue DHA levels. DHA feeding also caused a slight increase in phospholipid eicosapentaenoic acid (EPA). The increase in the tissue DHA and EPA levels was accompanied by a decrease in arachidonic acid levels. Dietary DHA resulted in a decrease in the specific activity of kidney microsomal Na+-K+ATPase from 508 +/- 70 to 261 +/- 25 ug PO4- min-1 mg protein-1 in mice consuming 4 g DHA per 100 g diet.
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