Cloning and expression of gene encoding P23 protein from Cryptosporidium parvum
We cloned the cp23 gene coding P23 (glyco)protein from Cryptosporidium parvum isolated from Thua Thien Hue province, Vietnam. The coding region of cp23 gene from C. parvum is 99% similar with cp23 gene deposited in NCBI (accession number: U34390). SDS-PAGE and Western blot analysis showed that the cp23 gene in E. coli BL21 StarTM (DE3) produced polypeptides with molecular weights of approximately 37, 40 and 49 kDa. These molecules may be non-glycosylated or glycosylated P23 fusion polypeptides. Recombinant P23 protein purified by GST (glutathione S-transferase) affinity chromatography can be used as an antigen for C. parvum antibody production as well as to develop diagnostic kit for C. parvum.
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