Preparation and assay of chalcone synthase from walnut tree tissue
Claudot, A.C. | Drouet, A.
Chalcone synthase activity was obtained from bark tissue of one-year-old shoots taken from a 30-year-old hybrid walnut tree Juglans nigra X J. regia. High tissue concentrations of phenolics, which are a major problem in woody plant enzyme extraction, were essentially overcome by the use of 200 mM ascorbic acid, 1.5% (w/v) polyethyleneglycol and 50 mM cysteine in the buffers, and a constant flow of nitrogen. Some properties of walnut tree chalcone synthase are discussed in comparison to those of the same enzyme from other plants.
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