Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis
2016
Mafu, Sibongile | Jia, Meirong | Zi, Jiachen | Morrone, Dana | Wu, Yisheng | Xu, Meimei | Hillwig, Matthew L. | Peters, Reuben J.
Significance Combinatorial biosynthesis is enabled by the use of promiscuous enzymes, but also can be applied to the discovery of such biocatalysts. Here, such an approach was used for the investigation of the substrate selectivity of cytochrome P450 enzymes from diterpenoid phytohormone biosynthesis, enabling facile characterization of both multiple substrates and structural analysis of the resulting products. The discovery of one such promiscuous cytochrome P450, reported here, provides insight into the underlying structure–function relationship and also may have biotechnological implications.
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