Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology
2020
FIRDOUS, Sadiqa | Iqbal, Samina | ANWAR, Samina
Glyphosate is an important organophosphonate herbicide used to eliminate grasses and herbaceous plants in many vegetation management situations. Its extensive use is causing environmental pollution, and consequently, there is a need to remove it from the environment using an eco-friendly and cost-effective method. As a step to address this problem, a novel bacterial strain Comamonas odontotermitis P2, capable to utilize glyphosate as a carbon (C) and/or phosphorus (P) source, was isolated from a glyphostate-contaminated field soil in Australia and characterized. Response surface methodology (RSM) employing a 2³ full factorial central composite design was used to optimize glyphosate degradation by C. odontotermitis P2 under various culture conditions. The strain C. odontotermitis P2 was proficient in degrading 1.5 g L⁻¹ glyphosate completely within 104 h. The optimal conditions for the degradation of glyphosate were found to be pH 7.4, 29.9 °C, and an inoculum density of 0.54 g L⁻¹ resulting in a maximum degradation of 90%. Sequencing of glyphosate oxidoreductase (GOX) and C-P lyase (phnJ) genes from C. odontotermitis P2 revealed 99% and 93% identities to already reported bacterial GOX and phnJ genes, respectively. The presence of these two genes in C. odontotermitis indicates its potential to degrade glyphosate through GOX and C-P lyase metabolic pathways. This study demonstrates the potential of C. odontotermitis P2 for efficient degradation of glyphosate, which can be exploited for remediation of glyphosate.
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