Cultural conditions promoting sclerotium formation in Stilbothamnium togoense
Wicklow, D.T. | Mcalpin, C.E.
Stilbothamnium togoense produced gram quantities of sclerotia on autoclaved oat [Avena sativa] seeds and oatmeal. Stipitate sclerotia (2-6 mm diam) developed on oat seeds while sessile sclerotia (1-20 mm diam; ave. = 10 mm) or poorly defined, flat, stromata-like tissues were produced on oatmeal. Many of the sessile sclerotia were 2-4 times larger than those previously reported for this species. Sclerotial yields (total dry weight) were twice as great for cultures incubated in continuous darkness than in cultures exposed to light. Maximum sclerotial yields were obtained at a substrate moisture content of 50-60%. In Petri dish cultures, maximum colony diameters (8 days) were obtained at temperatures of 25 or 28 C, depending on the culture medium used. No growth was recorded for cultures incubated at 10 or 32 C. Sclerotia were produced at 25 and 28 C but not at lower temperatures (15 and 20 C) at which growth occurred. Sclerotia from cultures incubated up to five months were examined for the presence of ascocarps, asci and ascospores but none were found.
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