Long-term Cytotoxicity Testing of the First Twenty MEIC Chemicals by the Determination of the Protein Content in Human Embryonic Lung Cells
1992
Dierickx, Paul J. | Ekwall, Björn
Within the framework of the MEIC (the multicentre evaluation of in vitro cytotoxicity) programme, the long-term cytotoxicity of the first twenty MEIC chemicals was investigated on MRC-5 cells. After treatment of the cells with each chemical for six weeks, the PI50 value was determined. The PI50 is the concentration (mM) of test compound required to induce a 50% reduction in total cell protein content. The results were compared with the acute PI50 values previously measured after treatment for 24 hours with the same compounds in Hep G2 cells. A good correlation between these two sets of data (r2= 0.94) was observed, with the exception of digoxin. Together with other preliminary MEIC validation results, this study indicates that a good alternative cytotoxicity test may be found for the prediction of human long-term toxicity.
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