Mitochondrial calcium uptake in bovine frozen sperm
1995
Beorlegui, N.B. | Cordoba, M. | Beconi, M.T.
Mitochondria from bovine frozen semen are capable of preserving oxidative energy. The purpose of this work was to study calcium uptake by the inner mitochondrial membrane which together with the plasmatic membrane, is involved in spermatic motility and viability regulation mitochondrial coupling was confirmed. In the active state, there was a drop in oxygen uptake rate using calcium concentrations ranging from 20 to 100 micromolar. In the resting state, 75 micromolar calcium concentration mimicked the active respiration state. In both states, concentrations greater than 100 micromolar induced mitochondrial uncoupling. Maximal mitochondrial uptake was recorded in the active state with 10 micromolar lactate. At calcium concentrations greater than 100 micromolar, there was a drop in mitochondrial calcium uptake for both respiration states. The ability to preserve oxidative energy allows the spermatozoon to take up mitochondrial calcium, thus regulating its functional activity.
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