Flaxseed Cyclolinopeptides: Analysis and Storage Stability
Aladedunye, Felix | Sosinska, Ewa | Przybylski, Roman
Fourteen cyclolinopeptides (CLPs) from flaxseed oil and meal were separated, identified and quantified by HPLC coupled to an Orbitrap MS. The oxidative stability of the cyclolinopeptides was assessed during storage of flaxseed oil and meal. A significant decrease in the amounts of the methionine containing CLPs, namely CLP-B, CLP-J and CLP-M, and a concurrent increase in the amounts of methionine sulfoxide containing CLPs, such as CLP-C, CLP-E and CLP-G were observed. The cyclolinopeptides with two methionine units, CLP-L and CLP-M, exhibited the greatest decrease, followed by CLP-J, the major flaxseed oil bitter taste precursor, and CLP-B, a biologically active cyclolinopeptide. At the end of the storage period, the amount of the bitter CLP-E increased fourfold in the oil while the immunosuppressive cyclolinopeptides A remained unchanged. No significant changes in the amount of each of the CLPs were observed in the stored flaxseed meals. A fast and reliable procedure has been developed for quantitative analysis of cyclolinopeptides. Due to the high predisposition of methionine containing cyclolinopeptides to oxidation and the easiness of CLPs’ quantification with the proposed method, it is possible to reliably assess the extent of flaxseed meal and oil oxidation.
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