Neospora caninum, a cause of abortion in donkeys (Equus asinus) in Iran
2022
Rahmani, Seyedeh Shabnam | Malekifard, Farnaz | Tavassoli, Mousa
Neospora caninum is an Apicomplexan parasite that can cause enormous economic losses due to abortions in cattle. The present study investigated the role of Neospora spp. infection in equine abortion in Iranian donkeys using molecular and phylogenetic analyses. Twenty-nine-aborted fetuses and 29 blood samples from their dams were collected from six different regions in the West and Northwest of Iran. They were tested for N. caninum by PCR at the Nc5 locus, followed by sequencing of five of the PCR products. The overall molecular prevalence was 34.5% in blood samples and the prevalence by DNA detection in the aborted fetuses was 13.8%. Evidence of transplacental transmission from positive jennies to their fetuses was detected in 40% of aborting jennies. Comparison of the five partial Nc5 sequences (227 bp length) exhibited 98–100% similarity with N. caninum GenBank sequences. This is the first molecular study and genetic characterization of N. caninum in Iranian donkeys suggests that N. caninum may be a significant cause of abortion in donkeys.
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