Quantitative determination of bovine caseinoglycomacropeptide in infant formulas by ultra‐high‐performance liquid chromatography‐electrospray‐ionization mass spectrometry
2011
Zhang, Jingshun | Ren, Yiping | Ma, Zhenyi | Huang, Baifen | Cai, Zengxuan | Li, Duo
An ultra‐high‐performance liquid chromatography‐electrospray ionization coupled to mass spectrometry method has been developed for determining caseinoglycomacropeptide (CGMP) in infant formulas by selected ion reaction and area monitoring modes. The present study focused on the optimization of sample pretreatment, chromatographic resolution and mass spectrometry parameters. After a simple sample pretreatment, the two genetic variants of caseinoglycomacropeptide, CGMPA and CGMPB, were separated using a BEH300 C18 column by gradient elution. The established method was extensively validated by determining the linearity (R2 >0.999), average recovery (95.8–118.4%), inter‐day precision (relative standard deviation ≤7.81%) and intra‐day precision (relative standard deviation ≤6.99%) based on two scan modes. To further verify the applicability of the method, 21 brands of commercial available infant formulas were analyzed. The results showed that the present method is selective, sensitive and reliable for separating and quantifying two genetic variants (CGMPA and CGMPB) of caseinoglycomacropeptide in infant formulas with complex matrix.
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