Capillary enzyme immunoassay with electrochemical detection for the determination of atrazine in water
1995
Jiang, T.B. | Halsall, H.B. | Heineman, W.R. | Giersch, T. | Hock, B.
A capillary enzyme immunoassay with electrochemical detection has been developed for the determination of atrazine in water. The assay is based on competitive binding between atrazine and alkaline phosphatase-labeled atrazine for a limited number of antibody binding sites. The antibody is attached covalently to a modified capillary surface. The enzymatic product (p-aminophenol) is detected by amperometric flow injection analysis. The calibration curve for atrazine has a linear range of 0.10-10.0 microgram/L and a detection limit of 0.10 microgram/L. An assay including six standards can be done in less than 60 min. Intra- and inter-assay precisions at 0.1 and 5.0 microgram/L are 9 and 8% and 6 and 10%, respectively. Recoveries of added atrazine from commercial bottled water, tap water, and Ohio River water at 0.50 and 5.0 microgram/L range from 100 to 115%. Simple filtration is the only step needed for sample cleanup.
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