Cytotoxicity of sulfurous acid on cell membrane and Bioactivity of Nitrosomonas europaea
2015
Jiang, Ruiyu | Wang, Mingqing | Xue, Jianliang | Xu, Ning | Hou, Guihua | Zhang, Wubing
Nitrosomonas europaea, an ammonia oxidizing bacterium, was chosen as a research model to study the alteration of cell membrane in the presence of sulfurous acid and biodegradation of acetochlor. Significant changes of the outer cell membrane were observed in the presence of sulfurous acid using scanning electron microscopy (SEM) and Atomic Force Microscopy (AFM). The fluorescence polarization has shown a significant decrease in membrane fluidity and the increase of permeability of cell membrane. Lysozyme experiment show the cell becomes easily influenced by substance in medium. Inductively Coupled Plasma–Mass Spectrometry (ICP–MS) measurements show considerable amount of Ca2+ and Mg2+ in the supernatant from the sulfurous acid exposed cells. Sulfurous acid treatment enhanced the ability of N. europaea to degrade acetochlor. On this basis, it can be concluded that the increased cell permeability is favor for the absorbability of nutrition. As a result, N. europaea grows faster and the biodegradation efficiency was improved.
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