A DNAzyme-based normalized fluorescence strategy for direct quantification of endogenous zinc in living cells
2022
Zhang, Xuanhao | Song, Zhi-Ling | Chao, Qiqi | Li, Qian | Kong, Rongmei | Fan, Gao-Chao | Luo, Xiliang
Taking the maximum fluorescence of an identical fluorophore as a reference, a DNAzyme-based normalized strategy is developed to unify the output signals under external interferences. This makes it possible to directly quantify endogenous zinc in living cells by in situ fluorescence imaging, implying promising potential in fundamental study and early disease diagnosis.
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