Transcriptome profiling analysis of two contrasting barley genotypes in general combining ability for yield traits
2021
Chen, Xiaodong | Zhao, Bin | Ji, Changhao | Zhu, Bin | Wang, Rui
General combining ability (GCA) has been successfully used to evaluate the parental performance in hybrid breeding program, while the molecular basis of GCA still remains unknown. In this study, RNA-Seq was employed to compare the transcriptome profiles that determined the GCA for yield-related traits in two different barley genotypes. A total of 1051 differentially expressed unigenes (DEGs) including 599 up-regulated DEGs and 452 down-regulated DEGs were found between Baodamai 12 and Ximala 22. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that many DEGs participated in metabolic process, stimulus response, catalytic activity and biosynthesis. Furthermore, 11 DEGs involved in the starch and sucrose metabolism pathway were discovered, among which eight up-regulated genes were related to beta-fructofuranosidase, pectinesterase, beta-glucosidase and hexokinase, while the other three down-regulated genes were related to sucrose-phosphate synthase, beta-glucosidase and hexokinase. These DEGs might contribute to the differences of GCA for grain weight between the two genotypes. The results may provide a new insight in the studying of the molecular basis of GCA and establish a foundation for further genomic research on the improvement of combining ability in barley breeding program.
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Эту запись предоставил National Agricultural Library