Evidence for the formation of glucose (not sucrose) in the metabolism of germinating sunflower seeds
Santos do Nascimento, R. | Seidl, P.R.
The application of carbon-13 nuclear magnetic resonance to studies of intact seeds has provided a technique for direct observation and determination of chemical constituents in living matter. A reexamination of products formed during the germination of sunflower, castor bean, and peanut seeds revealed that for the latter two sucrose is the main product from metabolism of germinating seeds, as expected. In the case of sunflower seeds, however, precise assignment of absorptions of individual carbon atoms reveals the presence of glucose and not sucrose, as was assumed from classical studies on mechanisms of germinating seed metabolism (this discrepancy is probably due to processes that occur during the extraction and labeling required for tracer studies). The results emphasize the quality of carbon-13 NMR spectra which can be obtained for mobile components of intact seeds during germination using a standard Fourier transform spectrometer.
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