Micronutrients zinc and boron enhance stevioside content in Stevia rebaudiana plants while maintaining genetic fidelity
2019
Singh, Pallavi | Dwivedi, Padmanabh
Micronutrients such as zinc (Zn) and boron (B) greatly affect plant growth and development. A pot experiment was conducted in garden soil in the poly house of the Horticulture garden, Banaras Hindu University, Varanasi, India to study the effect of zinc and boron application on stevioside content of Stevia plant. The data from two years (same season) showed that the foliar spraying of zinc sulphate (0.25, 0.50 and 0.75% w/v) and boric acid (0.25, 0.50 and 0.75% w/v) alone at all rates significantly increased stevioside content as compared to the control treatment. The most promising results were obtained from plants treated with 0.75% ZnSO4, as there was 30 times increase in the peak area noted in HPLC chromatogram. The in vitro raised plants were genetically stable as demonstrated using ISSR markers.
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