LEA proteins from Gastrodia elata enhance tolerance to low temperature stress in Escherichia coli
2018
Zeng, Xu | Ling, Hong | Yang, Jianwen | Li, Yuanyuan | Guo, Shunxing
Late embryogenesis abundant (LEA) proteins were initially discovered about 35years ago as accumulating late in embryogenesis of cotton seeds. Although abundant in seeds and pollens, these proteins have been found to protect cells against desiccation, cold, high temperature, and high salinity.Here, we present the first comprehensive survey of LEA proteins and their encoding genes in Gastrodia elata, a well-known medicinal orchid in China. Moreover, we researched on LEA family evolutionary relationships and functional characteristics.The LEA gene family in G. elata (GeLEAs) was cloned based on RNA-Seq data. In addition, all of GeLEA genes were introduced into Escherichia coli to assess the function of GeLEAs under low temperature stress.Based on the phylogenetic analysis with Arabidopsis and Oryza LEA proteins, we identified 8 LEA protein encoding genes in G. elata that could be classified into 6 distinct subgroups. The motif composition of these proteins was an important feature specific to LEA groups. Compared with control, the expressions of 5 GeLEAs in E. coli exhibited enhanced cold resistance and viability, indicating that GeLEAs protein could play a protective role in cells under low temperature stress.Our results suggest that LEAs from G. elata play an important role in responses to abiotic stress.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил National Agricultural Library