Ginsenoside production by hairy root cultures of Panax ginseng C.A. Meyer in bioreactors
2003
Yu, K.W. | Hahn, E.J. | Paek, K.Y.
Hairy roots infected with Agrobacterium rhizogenes were induced from roots, stems, and leaves of Korean ginseng (Panax ginseng C.A. Meyer) on half strength MS medium supplemented with 300 mg•L-1 Cefotaxim sodium. DNA extraction was carried out and PCR results confirmed that the hairy roots induced by A. rhizogenes KCTC 2703 have the rol C gene in their T-DNA. Selected root lines were propagated in 5-litre cone type bubble bioreactors containing MS media supplemented with 2.0 mg•L-1 NAA and 30 mg•L-1 sucrose. To increase ginsenoside content, jasmonic acid in various concentrations was added to the culture medium after 30 days of culture and the roots were then cultured for 7 more days until harvest. Total ginsenoside content increased with increasing jasmonic acid concentration, but high concentrations inhibited the root growth. Ginsenosid productivity was greatest at 2.0 mg•L-1 jasmonic acid. On the other hand, ginsenosides in the Rb group mainly increased, while those in the Rg group did not. Particularly, high concentrations (5 and 10 mg•L-1) of jasmonic acid decreased Rg1 content to almost half of that in the control, but significantly increased the Rb1 content of the Rb group. Among the ginsenosides in the Rb group, the Rb1 content increased more than Rb2, Rc, and Rd. There were gradual increases in ginsenosides in the Rb group, but those in the Rg group fluctuated then slightly decreased at the end of the culture period. Further studies are required to raise the contents of ginsenosides in the Rg group and to determine the optimal type of elicitors as well as optimal time for the elicitor treatment for accelerating ginsenoside production.
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