GISH analyses of backcross progenies of two Lilium species hybrids and their relevance to breeding
2012
Luo, J. R. | Ramanna, M. S. | Arens, P. | Niu, L.X. | Van Tuyl, J. M.
A total of 21 BC₁ progenies of Oriental Trumpet (OT) Lilium hybrids, nine BC₂ progenies of OT hybrids, and three BC₁ progenies of Martagon Asiatic (MA) hybrids were analysed using genomic in situ hybridisation (GISH). Of the BC₁ progenies of the OT hybrids, 15 were euploid (14 triploids, 2n = 3x = 36; 1 diploid, 2n = 2x = 24), and six were aneuploid. The triploid progenies had resulted from 2n eggs due to first division restitution (FDR). The aneuploid progenies had resulted from viable aneuploid gametes. All the BC₂ progenies of triploid (2n = 3x = 36) OOT hybrids were aneuploid. Chromosome numbers in the BC₂ progenies varied from 25 to 29. Two out of three BC₁ progenies of the MA hybrids were aneuploids, with 35 and 32 chromosomes, respectively. Another was a triploid (2n = 3x = 36) which had resulted from 2n eggs due to indeterminate meiotic restitution (IMR). GISH revealed extensive intergenomic recombination in the BC₁ progenies of OT and MA hybrids. A large number of Trumpet or Martagon chromosome segments were transmitted to the BC₁ progenies from the F₁ OT or MA hybrids. However, very few Trumpet chromosome segments were transmitted from the triploid BC₁ parent to the BC₂ progenies. These results indicate that it should be possible to select cultivars which possessed both traits from these BC₁ progenies and they would be fertile.
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