Heterotrimeric G protein γ subunit DEP1 is involved in hydrogen peroxide signaling and promotes aerenchyma formation in rice roots
2021
Chen, Yun | Chen, Yuanhua | Zhang, Yajun | Zhang, Dongping | Li, Guoming | Wei, Jiali | Hua, Xia | Lv, Bing | Liu, Lijun
Heterotrimeric G-protein α and β-subunits regulate H₂O₂-mediated aerenchyma formation. The rice G-protein γ-subunit, dense and erect panicle 1 (DEP1), is known to interact with the α-subunit and regulate nitrogen utilization and yield. However, it is unclear whether DEP1 regulates cell death for aerenchyma formation in rice roots. Using wild-type WYJ8 and its transgenic line WYJ8(DEP1), we confirmed that DEP1 is involved in H₂O₂-mediated aerenchyma formation. The rates of aerenchyma formation varied in different parts of the roots in both varieties, with the highest rate in the 4–7 cm segments, reaching a plateau in the 7–8 cm segments. Compared with WYJ8, the aerenchyma area and H₂O₂ content in WYJ8(DEP1) were increased by 55.98% and 53.37%, respectively; however, the responses of aerenchyma formation to exogenous H₂O₂ were basically the same in the two varieties. Diphenylene iodonium (DPI) treatment had no effect on H₂O₂ production and elimination processes in WYJ8, but significantly reduced the activity of the key enzyme that catalyzes H₂O₂ biosynthesis in WYJ8(DEP1). Importantly, exogenous H₂O₂ treatment did not offset the effect of the decrease in endogenous H₂O₂ level caused by DPI on aerenchyma formation. These results indicated that DEP1 enhanced H₂O₂ biosynthesis and promoted the cell death of the root cortex, thus contributing to aerenchyma development in WYJ8(DEP1).
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