Identification of structure and antioxidant activity of a fraction of polysaccharide purified from Dioscorea nipponica Makino
2008
Luo, D.
A large number of polysaccharides are present in boiling-water extraction of Dioscorea nipponica Makino. A DEAE-Sepharose CL-6B column chromatography was used to isolate the major polysaccharides from D. nipponica Makino. The largest amount of fraction of polysaccharide was subjected to further purification by gel-filtration on Sephadex G-100. The purified fraction was a neutral polysaccharide and a single peak in HPLC with Sugar KS-804 column, with a molecular weight of 38,000, and comprised mainly of glucose and fructose (45:1). Analysis by Periodate oxidation-Smith degradation indicated that there were 5.9%(1to)-glycosidic linkages, 4.94% (1 to 2)-glycosidic linkages, 61.16% (1 to 4)-glycosidic linkages, and 28% (1 to 3)-glycosidic linkages. On the basis of superoxide radical assay, hydroxyl radical assay, and self-oxidation of 1,2,3-phentriol assay, its antioxidant activity was investigated. This purified fraction of polysaccharide exhibited equivalent inhibiting power for self-oxidation of 1,2,3-phentriol to Vc, a little higher scavenging activity of superoxide radical and hydroxyl radical than Vc, and should be explored as a novel potential antioxidant.
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