Evaluation of a portable chlorophyll optical meter to estimate chlorophyll concentration in the green seaweed Ulva ohnoi
Masaló, Ingrid | Oca, Joan
In situ measurement of chlorophyll concentration, with an optical meter, in the green seaweed Ulva ohnoi is presented to estimate the absolute chlorophyll content. The MC-100 optical meter (Apogee Instruments) was used during the study; the optical meter gives chlorophyll content in relative units as Chlorophyll Content Index units (CCI). Absolute chlorophyll content, in μmol m⁻², was determined by extraction with dimethylformamide, and the chlorophyll concentration was measured with a spectrophotometer. Equations to convert CCI from optical meter to absolute chlorophyll content were presented for total chlorophyll, chlorophyll a, and chlorophyll b. Highly linear relationships (r² above 0.9, n = 130) were found for total chlorophyll, chlorophyll a, and chlorophyll b. In situ non-destructive estimation of U. ohnoi chlorophyll content using the MC-100 portable chlorophyll optical meter was considered convenient, fast, and accurate.
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