Rhizobium pakistanensis sp. nov., isolated from groundnut (Arachis hypogaea) nodules grown in rainfed Pothwar, Pakistan
2015
Khalid, Rabia | Zhang, Yu Jing | Ṣafdar, ʻAlī | Sui, Xin Hua | Zhang, Xiao Xia | Amara, Ummay | Chen, Wen Xin | Hayat, Rifat
A Gram-negative, white, non-motile, rod shaped bacterial strain BN-19ᵀwas isolated from a root nodule of groundnut (Arachis hypogaea) in Pakistan. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain BN-19ᵀformed a subclade in the genus Rhizobium together with Rhizobium alkalisoli CCBAU 01393ᵀ, Rhizobium vignae CCBAU 05176ᵀ, Rhizobium huautlense SO2ᵀand Rhizobium tarimense PL-41ᵀwith sequence similarities of 97.5, 97.3, 97.2 and 97.1 % respectively. Sequence analysis of housekeeping genes atpD, glnII and recA (with sequence similarities of ≤92 %) confirmed the unique position of BN-19ᵀin the genus Rhizobium. DNA–DNA relatedness between the strain BN-19ᵀand R. alkalisoli CCBAU 01393ᵀ, R. vignae CCBAU 05176ᵀ, R. huautlense SO2ᵀand R. tarimense PL-41ᵀwere 20.6, 22.5, 15.9 and 20.5 % respectively, further confirming that BN-19ᵀrepresents a novel species in the genus Rhizobium. The DNA G + C content was 60.1 mol%. The dominant fatty acids of strain BN-19ᵀwere C₁₉:₀cyclo ω8c, summed feature 2 (C₁₄:₀3OH and/or C₁₆:₁iso I) and summed feature 8 (C₁₈:₁ω7c). Some phenotypic features also differentiate the strain BN-19ᵀfrom the related species. On the basis of these results, strain BN-19ᵀis considered to represent a novel species in the genus Rhizobium, for which the name Rhizobium pakistanensis sp. nov. is proposed. The type strain is BN-19ᵀ(=LMG 27895ᵀ = CCBAU 101086ᵀ).
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