Optimization of Process Parameters for Directly Converting Raw Corn Stalk to Biohydrogen by Clostridium sp. FZ11 without Substrate Pretreatment
2016
Zhang, Jingnan | Sun, Huang | Pan, Chunmei | Fan, Yaoting | Hou, Hongwei
Statistical analyses had been used to optimize the process parameters of H₂ production from untreated raw corn stalk by Clostridium sp. FZ11. Five variables, including the temperature, initial pH, urea, phosphate-buffered saline (PBS), and nutrient solution, had been screened through the Plackett–Burman design. Experimental results indicated that urea, PBS, and nutrient solution were the most important variables, which were further optimized using the steepest ascent method and Box–Behnken design. The maximum H₂ yield of 96.2 mL/g from untreated raw corn stalk was recorded under the optimal conditions of 6.21 g/L urea, 0.19 M PBS, and 8.74 mL/L of nutrient solution. Meanwhile, about 45% of cellulose and 53% of hemicellulose were biodegraded by H₂ fermentation.
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