Metabolic profiling during germination of hydro primed cotton seeds
2019
Naguib, Deyala M.
This work tries to study the effect of the cotton seed hydropriming on its metabolic status during germination. Two groups of cotton seed (primed with water and non-primed) were germinated for 7 days. Through this period; the antioxidant enzymes, H2O2, Malonyl dialdehyde, lipid hydrolysis, shikimic acid, aldolase, and the isocitrate lyase were determined at 1st, 4th and 7th day of sowing. The study ensured that the hydropriming improved the germination of the cotton seed. Improved germination of primed seed combined with the enhancement of the stored lipid metabolism through the activation of lipase, the lipoxygenase and isocitrate lyase activity increased. The significant decrease in the shikimic acid content ensured the active metabolic status of the primed seed. Also priming increased considerably the antioxidant enzymes activity. This helped in maintaining the balance in the reactive oxygen species inside the primed germinated seed cells. This is illustrated in reducing H2O2 content in the primed germinated seed than that in the non-primed germinated seed. The control of reactive oxygen species in content helped in minimizing the lipid peroxidation in the primed germinated seed than that in the non-primed seed.
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