Cloning of the ANT gene and its expression profiles at different developmental stages and post-molting times in the ridgetail white prawn Exopalaemon carinicauda
Gao, Huan | Xue, Bei | Zhao, Lian | Lai, Xiaofang | Yan, Binlun | Cheng, Hanliang | Pan, Qian
To investigate the roles of the ANT gene, which codes for adenine nucleotide translocase (ANT) during crustacean development, a full-length cDNA sequence of EcANT in the ridgetail white prawn Exopalaemon carinicauda, was cloned, and its expression profile was analyzed at different developmental stages and post-molting times. The EcANT gene (GenBank accession number: KP892663) contained an open reading frame of 924 bp encoding a 307 amino acid protein with a theoretical size of about 33.42 kDa and a predicted isoelectric point of 9.77. Tissue expression analysis revealed that EcANT was mainly expressed in muscle and its expression level tended to increase with the developmental stages. In addition, the expression level of EcANT after molting increased following the lengthening of post-molting time. Our results suggest that EcANT is an important gene related to the growth and development of E. carinicauda.
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