Analysis of a rare recombination event within the multigenic Hor 2 locus of barley (Hordeum vulgare L.)
1990
Shewry, P.R. | Parmar, S. | Franklin, J. | Burgess, S.R.
A rare recombinant within the multigenic Hor 2 locus of barley was detected by SDS-PAGE of hordein fractions from F2 grain from the cross Bomi X P12/3. Analysis of a homozygous F4 line by, 2-D IEF/SDS-PAGE showed that recombination between the class I/II and class III subfamilies of genes had occurred, indicating that they are spatially separate within the Hor 2 locus. RFLP analysis using a B hordein-related cDNA clone confirmed that recombination had occurred, while similar analysis using a genomic clone related to gamma-type hordein encoded by the Hor 5/HrdF locus) indicated the order of the two subfamilies of genes on the short arm of chromosome 5, the class I/II genes being closer to the centromere. The results are consistent with the origin of the B hordein gene family from initial duplication of a single ancestral gene to give two genes which diverged to become the ancestors of the class I/II and class III subfamilies. Subsequent cycles of duplication and divergence have resulted in the present high degree of polymorphism.
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