Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium?
Clemente, M. | Fuente, J. de la | Fair, T. | Al Naib, A. | Gutierrez-Adan, A. | Roche, J.F. | Rizos, D. | Lonergan, P.
The steroid hormone progesterone (P₄) plays a key role in the reproductive events associated with pregnancy establishment and maintenance. High concentrations of circulating P₄ in the immediate post-conception period have been associated with an advancement of conceptus elongation, an associated increase in interferon-τ production and higher pregnancy rates in cattle. Using in vitro and in vivo models and ~8500 bovine oocytes across six experiments, the aim of this study was to establish the route through which P₄ affects bovine embryo development in vitro and in vivo. mRNA for P₄ receptors was present at all stages of embryo development raising the possibility of a direct effect of P₄ on the embryo. Exposure to P₄ in vitro in the absence or presence of oviduct epithelial cells did not affect the proportion of embryos developing to the blastocyst stage, blastocyst cell number or the relative abundance of selected transcripts in the blastocyst. Furthermore, exposure to P₄ in vitro did not affect post-hatching elongation of the embryo following transfer to synchronized recipients and recovery on Day 14. By contrast, transfer of in vitro derived blastocysts to a uterine environment previously primed by elevated P₄ resulted in a fourfold increase in conceptus length on Day 14. These data provide clear evidence to support the hypothesis that P₄-induced changes in the uterine environment are responsible for the advancement in conceptus elongation reported previously in cattle and that, interestingly, the embryo does not need to be present during the period of high P₄ in order to exhibit advanced elongation.
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