Structural characteristics of purified glycinin from soybeans stored under various conditions
2004
Hou, D.H.J. | Chang, S.K.C.
Soybeans were stored in 84% relative humidity at 30 °C (adverse conditions) for 9 months and in 57% relative humidity at 20 °C, cold (4 °C), and an uncontrolled ambient garage for 18 months. Glycinin was isolated and purified; its structural properties were characterized. The purified glycinin from soybean in the adverse conditions was associated with a significant amount of sugar and showed reductions in hydrophobic interactions after 3 months; the total free sulfhydryl content in glycinin decreased, but the intramolecular disulfide bonds increased; the α-helix content of secondary structure slightly increased, but the β-sheet content decreased. The structure of glycinin purified from the other three conditions showed no significant changes for 18 months of storage when compared to the control. The molecular mass of glycinin remained in the range of 313-340 kDa during the whole storage period for the four conditions.
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