Differentiation of porcine wild-type lactobacilli strains, with ERIC-PCR and PFGE band patterns included in polyphasic taxonomy
Bolado-Martínez, E. | Acedo-Félix, E.
Probiotic administration is an alternative to antibiotic supplementation in pig management. However, potential probiotic microorganisms included in foods must be well defined and correctly named, on the basis of a valid taxonomic system. The aim of the present study was to analyze band patterns generated by the polymerase chain reaction (PCR) amplification of enterobacterial repetitive intergenic consensus sequences (ERIC-PCR), obtained from porcine wild-type <i>Lactobacillus</i> strains, as a rapid alternative for genotypic characterization in polyphasic taxonomy. In the present study 36 porcine wild-type <i>Lactobacillus</i> strains were analyzed by polyphasic taxonomy which included API 50 CHL system, ERIC-PCR, and pulsed field gel electrophoresis (PFGE) band analysis, after digestion with <i>XbaI</i> or <i>SpeI</i> restriction enzymes. Polyphasic taxonomy discriminated among 23 strains of <i>Lactobacillus reuteri</i>, 12 strains of <i>Lactobacillus salivarius</i> and one strain of <i>Lactobacillus mucosae</i>. None of the tested methods was able to reliably resolve the three selected species of lactobacilli at a strain level. However, results improved considerably when ERIC-PCR results were combined with phenotypic characterization, and those results were comparable to the taxonomy that included PFGE.
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