Demonstration of increased amounts of apolipoprotein B in intestinal epithelium after fat feeding
1979
A very few people suffer a rare genetic disease known as abetalipoproteinemia, characterized by fat malabsorption, acanthocytic erythrocytes, lack of plasma lipoproteins (chylomicrons, VLDL and LDL) containing apolipoprotein B (apoB), and large lipid droplets in intestinal epithelial cells. A comparison of intestinal biopsies on 5 normal subjects and 2 patients with abetalipoproteinemia was made after fasting and after ingesting 200 ml of cream, utilizing the techniques of lipid staining or immunofluorescence. In normal subjects, small lipid droplets were found in the apical part of the cell after fasting, which increased greatly after eating cream, as did apoB. No apoB was detected in patients either before or after the fat feeding; their biopsies revealed large lipid drops in greater amounts than the cells of normal subjects. It is possible that there is an immunologically altered form of apoB present, but it would be incapable of forming chylomicron.
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