Fermentation studies on roselle (Hibiscus sabdariffa) calyx neutralized with trona (sodium sesquicarbonate) at different concentrations
2010
Ojokoh, A. O.
The effect of trona at different concentrations on the fermentation of roselle (Hibiscus sabdariffa) calyx was studied. Mixed flora of bacteria and fungi were isolated and identified from the fermenting calyx. The isolates were Micrococcus luteus, Staphylococcus aureus, Echerichia coli, Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger and A. flavus. The addition of trona to the samples raised the initial pH which affected the terminal pH. There was an increase in microbial population with increases in the concentration of trona, especially for bacterial population which ranged from 5.18 to 5.59 log10 c.f.u./ml. The changes in total titratable acidity level were inconsistent. Fermentation enhanced the protein content of the samples but significantly decreased (P < 0.05) the content of antinutritional factors (phytic acid and tannin).
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