Rapid clonal propagation of Dioscorea zingiberensis
2003
Chen, Y. | Fan, J. | Yi, F. | Luo, Z. | Fu, Y.
A protocol was developed for rapid in vitro propagation of Dioscorea zingiberensis Wright using stems as explants. MS medium with the macroelements at half strength and supplemented with 20.0 g l-1 sucrose and 8.0 g l-1 agar was used as basal medium. Lateral buds on nodal cuttings grew into shoots within 20 days after culture on basal medium supplemented with 4.4 micromolar 6-benzylaminopurine (BAP) and 1.1 micromolar α-naphthalene acetic acid (NAA). The shoots were cut into segments and cultured on medium with 8.9 micromolar BA and 5.4 micromolar NAA for 30 days for callus formation. The callus was cut into pieces and cultured on medium containing 22.2 micromolar BAP and 1.1 micromolar NAA, on which 87.5% of the callus pieces regenerated multiple shoots within 50 days. The shoots were rooted on medium containing 4.9 micromolar indole-3-butyric acid (IBA) for 20 days. Adventitious buds and shoots could be repeatedly formed after the calli were cut into pieces and cultured on the medium containing 8.9 micromolar BAP plus 1.1 micromolar NAA. More than 85% of the regenerated plantlets survived and grew vigorously 1 month after they were transplanted in vermiculite and each plant formed 2-4 microtubers 3 months of transplanting.
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