A predictive model of growth from spores of non-proteolytic Clostridium botulinum in the presence of different CO2 concentrations as influenced by chill temperature, pH and NaCl
2001
Fernandez, P.S. | Baranyi, J. | Peck, M.W.
In the present study, the effect of modified atmospheres ranging from 0-90% CO2 (balance 10% H2 and N2) was evaluated on growth from spores of non-proteolytic Clostridium botulinum (types B, E and F), at different pH and NaCl concentrations, over the temperature range 5-12 degrees C. Growth tests were carried out using a model broth system, PYGS (peptone, yeast, glucose, starch). In general terms, a CO2 concentration of 5% enhanced growth, 50% CO2 showed a moderate inhibition and 90% had a considerable inhibitory effect. A predictive model has been developed that describes the effect of CO2, in combination with other mild preservative factors, on growth from spores of non-proteolytic C. botulinum. The model provides a good description of the experimental data.
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