Laccase isozymes: production by an opportunistic pathogen, a Fusarium proliferatum isolate from wheat
2001
Kwon, S.I. | Anderson, A. J.
Laccases often are present in plant tissues colonized by fungal pathogens. Non-denaturing gel electrophoresis revealed laccase isozymes in extracts of wheat leaf sheaths infected by Fusarium proliferatum (Fp). No laccase isozymes were detected in extracts of healthy wheat. The four anionic isozymes (LacA-A to A-D) in the infected wheat extracts corresponded to those produced by the Fp isolate when cultured on low carbon medium. Only two of these isozymes (LacA-C and A-D) were found in the culture filtrates. A single anionic laccase isozyme (LacA-B) was detected on nitrogen-limited medium. Other cationic isozymes produced in vitro were not detected from the diseased sheath tissue. Northern hybridization revealed expression of a transcript corresponding to a potential laccase-gene sequence LacRT-1 in planta and in vitro under conditions of low carbon and nitrogen supply.
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