Culture-Independent Analysis of Fecal Microbiota in Cattle
OZUTSUMI, Yuhei | HAYASHI, Hidenori | SAKAMOTO, Mitsuo | ITABASHI, Hisao | BENNO, Yoshimi
The phylogenetic diversity of the fecal bacterial community in Holstein cattle was determined by 16S ribosomal RNA gene sequence analysis. The sequences were affiliated with the following phyla: Firmicutes (81.3%), Bacteroidetes (14.4%), Actinobacteria (2.5%), and Proteobacteria (1.4%). The Clostridium leptum subgroup was the most phylogenetically diverse group in cattle feces. In addition, a number of previously uncharacterized and unidentified bacteria were recognized in clone libraries.
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