Global Profiling and Structural Characterization of Metabolites of Ononin Using HPLC-ESI-IT-TOF-MSⁿ After Oral Administration to Rats
2020
Li, Hong-Fu | Li, Teng | Yang, Ping | Wang, Yong | Tang, Xue-Jian | Liu, Li-Jia | Xu, Feng | Shang, Ming-Ying | Liu, Guang-Xue | Li, Yao-Li | Wang, Xuan | Yin, Jun | Cai, Shao-Qing
Ononin is a bioactive isoflavone of legumes. To explore the “effective forms” of ononin, its metabolites were characterized using HPLC–ESI-IT-TOF-MSⁿ after oral administration to rats. Metabolites (106), including 94 new metabolites, were characterized, which contained 17 phase I, 23 hydroxylated and methylated, 54 sulfated, 10 glucuronidated, and 2 sulfated and glucuronidated metabolites. Six hydroxylated metabolites of formononetin (aglycone of ononin) were simultaneously detected for the first time. Twenty-three hydroxylated and methylated metabolites were the new metabolites of ononin, and the number of hydroxylation and methylation was 1–3 and 1–2. Twenty metabolites have ononin-related bioactivities, and many metabolites have the same bioactivities. Their probable mechanisms of action may be additive and/or synergistic effects, especially because of the addition of the blood concentrations of these compounds. The results provide a foundation for a better understanding of the “effective forms” of ononin.
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