Control of root-knot nematode with formulations of the nematode-trapping fungus Arthrobotrys dactyloides
Stirling, G.R. | Smith, L.J. | Licastro, K.A. | Eden, L.M.
Arthrobotrys dactyloides grew readily in shaken flasks containing glucose corn steep powder and 8-10 g dry wt of fungal biomass/liter medium was usually produced in 5-6 days. However, it was difficult to convert this biomass into a viable, granulated product suitable for commercial use in biological control. Formulations prepared using kaolin and vermiculite as carriers and gum arabic as a binder showed poor viability when biomass was harvested from liquid culture, mixed with formulation ingredients, granulated, and then dried to a moisture content of less than 5%. Inclusion of a solid-phase incubation step following granulation and prior to drying (incubation of moist granules for 3 days at 25 degrees C in a sterile plastic bag aerated with sterile air) markedly improved biological activity. When granules produced in this manner were placed on a glass slide in field soil, hyphae proliferated from granules and always produced traps. Seven experiments in soil microcosms showed that formulations which had been subjected to solid phase incubation prior to drying consistently reduced numbers of Meloidogyne javanica juveniles by more than 90%. In seven glasshouse experiments in which field soils were treated with granules (10 g/liter) and planted to tomatoes, the number of galls induced by the nematode was reduced by 57-96%.
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